Description
This used NanoTemper Monolith NT.115 Pico MST System is a MicroScale Thermophoresis instrument designed for sensitive analysis of biomolecular interactions in solution. The system is suitable for binding affinity studies, protein-protein interactions, protein-small molecule interactions, protein-nucleic acid interactions, antibody/antigen studies, enzyme assays, buffer screening and biophysical assay development.
The Monolith NT.115 Pico uses MicroScale Thermophoresis to detect changes in molecular movement in a microscopic temperature gradient. Binding events can be measured directly in capillaries with low sample consumption and without surface immobilization. The Pico configuration is especially suited for high-sensitivity MST measurements with red fluorescent labels such as NT-647, Alexa Fluor 647, Cy5 and compatible dyes.
The system is supplied with a Windows 10 PC with NanoTemper NTControl v2.1.31 installed and configured for instrument control and measurement acquisition. The additional NanoTemper MO.Affinity Analysis software enables advanced evaluation of the recorded MST data, generation of binding curves and determination of binding affinities (Kd). Two NT.115 capillary trays, capillaries and additional accessories are included as pictured.
Specifications
- Manufacturer: NanoTemper Technologies GmbH
- Model: Monolith NT.115 Pico
- Type: MicroScale Thermophoresis system / MST instrument
- Serial number: 201403-BR-P001
- Manufacturing date: 03/14/2014
- Technology: MicroScale Thermophoresis
- Detection configuration: Pico / high-sensitivity red fluorescence MST detection
- Suitable fluorophores: NT-647, Alexa Fluor 647, Cy5 and compatible red fluorescent labels
- Measurement format: glass capillaries
- Number of capillaries per run: up to 16
- Typical sample volume per capillary: approximately 4–5 µL
- Affinity range: pM to mM range, depending on assay, sample and fluorophore
- Applications: binding affinity, Kd determination, protein interactions, small molecule binding, antibody/antigen binding, nucleic acid interactions, aggregation studies, buffer screening and assay development
- Temperature control: approximately 22°C to 45°C
- IR laser wavelength: 1475 nm ± 15 nm
- IR laser power: 120 mW max.
- Laser classification: Class 1 laser product
- Display: integrated touchscreen / status display
- Computer: Windows 10 PC included
- Installed software: NanoTemper NTControl V2.1.31 and MO. Affinity Analysis software.
- Connections: Ethernet and USB visible
- Electrical input: 12 V DC
- Maximum current input: 5.0 A
- Power supply: external 12 V DC power supply required
- Dimensions W x H x D: approximately 33 x 45 x 51 cm
- Weight: approximately 24 kg
- Country of origin: Germany
- Included accessories: 2 x NT.115 capillary trays as pictured
- Included consumables: Monolith NT.115 capillaries, including Standard Treated and Premium Coated capillaries as pictured
- Included reagents / buffers: assorted NanoTemper labeling reagents, BSA, Tween-20, MST buffer and control buffers as pictured
- Additional items: USB cable and accessories as pictured
- Estimated original new price: approximately €100,000–€120,000 depending on configuration, detector, software, accessories and service package
