Description
This used NanoTemper Monolith NT.115 Pico MST System is a MicroScale Thermophoresis instrument designed for sensitive analysis of biomolecular interactions in solution. The system is suitable for binding affinity studies, protein-protein interactions, protein-small molecule interactions, protein-nucleic acid interactions, antibody/antigen studies, enzyme assays, buffer screening and biophysical assay development.
The Monolith NT.115 Pico uses MicroScale Thermophoresis to detect changes in molecular movement in a microscopic temperature gradient. Binding events can be measured directly in capillaries with low sample consumption and without surface immobilization. The Pico configuration is especially suited for high-sensitivity MST measurements with red fluorescent labels such as NT-647, Alexa Fluor 647, Cy5 and compatible dyes.
The instrument powers on and reaches the instrument status screen. The display shows connected status and software/firmware version 1.2.1. The system is supplied with two NT.115 capillary trays, capillaries and assorted NanoTemper reagents / buffers as pictured. Software can also be supplied by arrangement. The system is supplied as pictured.
Specifications
- Manufacturer: NanoTemper Technologies GmbH
- Model: Monolith NT.115 Pico
- Type: MicroScale Thermophoresis system / MST instrument
- Serial number: 201403-BR-P001
- Manufacturing date: 03/14/2014
- Technology: MicroScale Thermophoresis
- Detection configuration: Pico / high-sensitivity red fluorescence MST detection
- Suitable fluorophores: NT-647, Alexa Fluor 647, Cy5 and compatible red fluorescent labels
- Measurement format: glass capillaries
- Number of capillaries per run: up to 16
- Typical sample volume per capillary: approximately 4–5 µL
- Affinity range: pM to mM range, depending on assay, sample and fluorophore
- Applications: binding affinity, Kd determination, protein interactions, small molecule binding, antibody/antigen binding, nucleic acid interactions, aggregation studies, buffer screening and assay development
- Temperature control: approximately 22°C to 45°C
- IR laser wavelength: 1475 nm ± 15 nm
- IR laser power: 120 mW max.
- Laser classification: Class 1 laser product
- Display: integrated touchscreen / status display
- Software platform: MO.Control / MO.Affinity Analysis workflow
- Software availability: software can be supplied by arrangement
- Connections: Ethernet and USB visible
- Electrical input: 12 V DC
- Maximum current input: 5.0 A
- Power supply: external 12 V DC power supply required
- Dimensions W x H x D: approximately 33 x 45 x 51 cm
- Weight: approximately 24 kg
- Country of origin: Germany
- Included accessories: 2 x NT.115 capillary trays as pictured
- Included consumables: Monolith NT.115 capillaries, including Standard Treated and Premium Coated capillaries as pictured
- Included reagents / buffers: assorted NanoTemper labeling reagents, BSA, Tween-20, MST buffer and control buffers as pictured
- Additional items: USB cable and accessories as pictured
- Estimated original new price: approximately €100,000–€120,000 depending on configuration, detector, software, accessories and service package
