The offered used Waters nanoACQUITY UPLC system supplies direct (non-split) capillary and nanoflow rates for high-resolution chromatographic separations optimized for columns ranging from 1 mm to 75 μm internal diameter. The system originates from an operational laboratory and was last serviced in november 2016.
NanoACQUITY UPLC systems are often used in proteomics and biomarker discovery were scientests must obtain the most information from very small amounts of highly complex, often irreplaceable samples. The direct nano-flow design of the nanoACQUITY UPLC system run without a flow-splitter, removing the need to adjust fittings and perform flow calibrations just to change the flow rate. With this easy-to-use system, you’ll see better robustness and chromatographic reproducibility. Break through the limitations of your current nano-scale HPLC system and step into a world of information-rich analyses your laboratory can count on.
The system consists of the following elements:
- Acquity Binary Solvent manager: The nanoACQUITY binary solvent manager is a high-pressure pump that moves solvent through the system. It provides steady (pulse-free) solvent flow at flow rates ranging from 0.20 to 5.00 μL/min (under closed loop control) or to 100.0 μL/min (under open loop control) at 69,000 kPa (690 bar, 10,000 psi). The solvent manager can generate high-pressure gradients with minimal gradient delay.
- Acquity Sample manager with integrated heating and trapping module: The sample manager injects the samples it draws from microtiter plates, or vials, into the chromatographic flow stream. In maximum throughput mode, the sample manager can perform an injection in approximately 45 seconds, including a single default wash, or it can do so in less than 60 seconds, including a dual default wash. The sample manager accepts standard footprint plates, 5.03 ±0.02 inch × 3.365 ±0.02 inch, that conform to SBS/ANSI-compliant plates (maximum height of 2.2 inches, including covers). You may program any combination of plates and vial holders for automated sample processing. Samples are loaded into the sample manager via the front door. The sample manager can maintain samples at any temperature between 4 and 40 °C (39 and 104 °F) in ambient conditions of 25 °C (77 °F) or less.
- Single oven column heater with trapping valve
- Optionally an auxiliary solvent manager and FlexCart can be added to this system. These modules are not part of this offer or in stock.
- Maximum resolution – Optimized system to drive 1.7 μm BEH particle technology to yield maximum peak capacity for complex mixtures.
- Maximum reproducibility – Direct nanoflow control and a novel, moveable heating and trapping module for < 0.25-minute standard deviation run-to-run reproducibility, even over long gradients.
- Maximum throughput – BEH Technology columns enable faster gradient separations for simple mixtures, or when preforming targeted analyses without compromising resolution.
- Robustness – Nano-scale columns and fittings (PEEK, 1/32 stainless steel and gold coating) provide reliable operation for up to 10,000 psi, with novel high pressure trapping.
- Ease-of-use – Direct gradient control enables minimized tubing assemblies and simplified loading and trapping schemes.
- Enhanced on-board diagnostics – Efficiently monitor system performance.
- Combine with any MS – From Waters’ Quattro Premier XE tandem quadrupole mass spectrometer, for routine quantification to the Synapt™ High Definition MS (HDMS™) system for cutting edge applications, the nanoACQUIT Y UPLC system is the only inlet you’ll need for high-sensitivity nano-scale MS analyses. In addition the nanoACQUITY UPLC system can be controller from your Thermo Fisher Scientific, or ABI/MDS SCIEX mass spectrometer software platforms
- Advanced software – By matching our nano-scale UPLC/MS system solutions with MassLynx™ software for specialized data processing, you will complete your analysis accurately, quickly and with complete confidence in your answers.